Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TBE Urea Running Buffer (10X), 1 L
Product DescriptionTBE(Tris-Borate-EDTA) urea running buffer is used for protein analysis. It has a 10X concentration and contains 0.89M Tris base, 0.89M boric acid, 20 mM EDTA, and a pH at 8.3.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Medchemexpress LLC NVP-TAE684 | 761439-42-3 | MFCD11977634 | 99.7% | 614.2 g/mol | C30H40ClN7O3S | 10 MG
NVP-TAE684 is a potent, selective small-molecule inhibitor of anaplastic lymphoma kinase (ALK) used in biochemical and cellular research. It blocks growth of ALK-dependent and ALCL-derived cell lines with low-nanomolar IC50 values and has been applied in preclinical studies to probe ALK signaling and validate target engagement. Typically supplied as a solid for in vitro experiments.
- Potent ALK inhibition at low-nanomolar concentrations.
- Selective activity against ALK-driven cell lines.
- Suitable for biochemical and cellular assays.
- Supplied as a solid for research use.
- Characterized with reported purity and analytical data.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005335 HEPES EMPROVE EVOLVE 100G
Medchemexpress LLC 1-Piperazinepropanesulfonic acid, β-hydroxy-4-(2-hydroxyethyl)- | 68399-78-0 | 99.9% | 50 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It has a pKa of 7.84 at 2.0 mM buffer concentration and exhibits a relatively high ability to bind copper(II). It is for research use only.
- Zwitterionic buffer
- Working pH range: 7.1-8.5
- pKa of 7.84 at 2.0 mM buffer concentration
- Relatively high ability to bind copper(II)
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 100 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255310.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 100 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
Perkin Elmer US LLC ELAN 9000/6X00/DRC-e Wash Solution, 1% HNO3, 1,000 mL
Concentration of Element includes: Matrix Volume 1 % HNO3 1,000 mL
Hampton Research HR2-902-12/StockOptions HEPES #12 pH 7.9, 185 ml
HR2-902-12/StockOptions HEPES #12 pH 7.9, 185 ml. Please note items from this supplier are non-returnable.
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660049.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 5 Kg
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid), 5 kg, is a large-volume biochemical reagent used for pH buffering in various biochemical and biological experiments. This high-quality MES buffer maintains a stable pH environment, essential for sensitive reactions such as protein assays, enzyme catalysis, and cell culture work. The 5 kg volume is ideal for laboratories requiring bulk amounts of MES for extended projects or regular use in high-throughput testing. MES is widely recognized for its minimal interference with biological systems, ensuring it does not alter the outcomes of complex experiments. It is commonly used in molecular biology, biochemistry, and biotechnology.