Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific HEPES Buffered Saline Solution 2X, 250 Milliliters
HEPES Buffered Saline Solution 2X is a highly stable buffered solution commonly used in biological research and laboratory applications. It is formulated with HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid) to maintain a stable pH in biological systems. The 250mL bottle contains a 2X concentrated solution that can be diluted to the required working concentration. HEPES Buffered Saline is widely used in cell culture, protein studies, and molecular biology applications, providing a stable environment for maintaining cell viability and pH stability during experiments. This solution is ideal for biological assays, enzyme studies, and other sensitive applications requiring precise pH control.
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 500g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 500g
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 25g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 25g
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
![Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661260.jpg-250.jpg)
Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 100 G
MOPSO (3-(N-Morpholino)2-hydroxypropanesulfonic acid) sodium salt is a specialized pH buffer used in biochemical applications where precise pH control is necessary. This 100g volume of MOPSO sodium salt is ideal for laboratories that require small quantities of this specific buffer for experiments that involve enzymatic reactions, protein assays, or cell culture applications. MOPSO offers a high degree of buffering capacity in the pH range of 7.0–7.5, making it suitable for a variety of molecular biology protocols. Its stability at this pH range is ideal for experiments where small fluctuations in pH can significantly affect the outcome. MOPSO is particularly valuable in applications that require precise control of the pH environment.
ILEX LIFE SCIENCES LLC PAN-Biotech HEPES Buffer 1M (100 ml)
HEPES Buffer 1M. QUANTITY: 100 ml. MANUFACTURED BY: Pan-Biotech (Germany). STORAGE: 2 to 8 degrees Celsius. DESCRIPTION: HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent; one of the twenty Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by aerobic respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. COMPOSITION: HEPES 238.31 g/L.
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Bio Rad Laboratories XT MES RUNNING BUFFER 500ML
XT MES Running Buffer, 20x, 500mL, Liquid, White, Odorless, Boiling Point: 100degreeC, pH: 6.4 at 20degreeC, Fully miscible in water, No special requirements for storage
Sigma Aldrich Fine Chemicals Biosciences PIPES disodium salt >=99% (titration) | 76836-02-7 | MFCD00064349 | 1KG
PIPES disodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 346.33 | 76836-02-7 | MFCD00064349 | 1KG
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 500G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500G
Medchemexpress LLC 4-Morpholineethanesulfonic acid, hydrate | 1266615-59-1 | 99.9% | 25 G
4-Morpholineethanesulfonic acid, hydrate | 1266615-59-1 | 99.9% | 25 G
Teknova 1M HEPES, pH 7.5, 20L Bag, Sterile. 4 Pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M MOPS Buffer, pH 7.4, 1000mL, Sterile. 2 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.