Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml
HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml. Please note items from this supplier are non-returnable.
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 20ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (20 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 100ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (100 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Boston Bioproducts Inc Phosphate Buffered Saline-Tween (PBST), 0.05% Tween-20, pH 7.4, 7.0 - 7.4 buffer range, ready-to-use, immunoassays, ELISA, Western Blot, protein purification
Phosphate Buffered Saline-Tween (PBST), 0.05% Tween-20, pH 7.4, 7.0 - 7.4 buffer range, ready-to-use, immunoassays, ELISA, Western Blot, protein purification
Bioworld ACE MES Buffer, 50mM, pH 6.0, 250 mL
Product DescriptionA.C.E.™ MES (compound 2-(N-morpholino)ethanesulfonic acid) buffer has a concentration of 50 mM with a pH level of 6.0. The buffer is biotechnology grade and is formulated for capillary electrophoresis. Buffer is made with 3D water and filtered through 0.22 µ filter. Product is shipped at room temperature.
Bioworld Tris-Glycine Sample Buffer (4X), 25 mL
Product DescriptionTris-Glycine (TG) sample buffer has a concentration of 4X. It contains 400 mM Tris-HCl at a pH of 8.6, 40% glycerol, and 0.02% bromophenol blue.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Chem-Impex International, Inc. Bicine | 150-25-4 | MFCD00004295 | 100G
Bicine, 150-25-4, MFCD00004295, 100G
Polysciences, Inc. Tris Buffered Saline; Composition: 1M Tris-HCl, 3M Sodium chloride; 8.0+/-0.2 pH; Storage: 25 deg.C
Tris Buffered Saline; Composition: 1M Tris-HCl, 3M Sodium chloride; 8.0+/-0.2 pH; Storage: 25 deg.C
Boston Bioproducts Inc Glycine Buffer in PBS (0.2 M, pH 2.5) - 500ml
The Glycine Buffer in PBS is widely used in various laboratory applications such as polyacrylamide gel electrophoresis as a running buffer, a transfer buffer for Western blotting, enzyme assays, and cell culture. This Buffer type serves as a tool for maintaining stable conditions during biochemical experiments in the pH range of 2.2 to 3.6. The Glycine buffer is an essential part of many types of protein purification procedures, including the purification and recovery of monoclonal antibodies.
Sciencell Research Laboratories DULBECCO'S PHOSPHATE-BUFFERED
Dulbecco's Phosphate-Buffered Saline 500 ml
Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.
National Diagnostics TBE BUFFER (10X) 1 LT
TBE Buffer (10X), Formulated with 18MegOhm water. 0.2 micron filtration. The most stable TBE on the market, 1 LT
Sigma Aldrich Fine Chemicals Biosciences Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 6X1L
Hanks' Balanced Salt solution Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture | 6X1L
West Pharmaceutical Services Inc WEST PHARMACEUTICAL SERVICES INC
NC3936200 V-35 4432/50G SIL 1000/CS